Gene Editing / CRISPR Core

 CRISPR technology facilitates the precise editing of targeted regions of mammalian genomes and remains one of the key recent advances in biotechnology.

The CRISPR Technology Core (CTC) located within the ICBR, offers UFHCC researchers with access to expertise in Cas9-mediated gene engineering in mammalian cells as well as custom services for CRISPR-based whole-genome phenotypic screens.

The CTC is under the direction of Dr. Chris Vulpe (CTHR) who provides free consultation to UFHCC members. Services can be accessed using the ICBR iLab web portal.

 

ICBR Gene Expression & GenotypingCRISPR ServicesAcademic  Non-Profit Commercial
ICBR Gene Expression & GenotypingCRISPR Project consultation (1 hour)$0.00$0.00 $0.00
ICBR Gene Expression & GenotypingCRISPR Project consultation (additional hours)$50.00$57.50$62.50
ICBR Gene Expression & GenotypingCell Line Optimization for Transduction or Nucleofection$50.00$57.50$62.50
ICBR Gene Expression & GenotypingCRISPR Single Cell KO in Cell Line Pool Using 1 sgRNA$400.00$460.00$500.00
ICBR Gene Expression & GenotypingCRISPR Single Cell KO in Cell Line Pool Using 3 sgRNAs (Recommended)$1,000.00$1,150.00$1,250.00
ICBR Gene Expression & GenotypingGeneration of CRISPR Single Gene KO Stable Cell Line Using 3 sgRNAs [3 Clonal Isolates per Gene]$2,500.00$2,875.00$3,125.00
ICBR Gene Expression & GenotypingVector-Free CRISPR Single Gene KO Stable Cell Line Using Nucleofection of Cas9 Protein and 3 sgRNA [3 Clonal Isolates]$2,100.00$2,415.00$2,625.00
ICBR Gene Expression & GenotypingVector-Free CRISPR Single Gene KO Cell Line Pool Using Nucleofection of Cas9 Protein and 1 sgRNA [3 Clonal Isolates]$700.00$805.00$875.00