Drug Screening Core

The Drug Screening Core (DSC) has the capability to run medium- to high- throughput screens (96- and 384-well) and experienced personnel to miniaturize assays. Assay platforms include biochemical assays using colorimetric, fluorescence and ultra-sensitive luminescence outputs including assays designed to quantify protein concentration and protein-protein interactions using the AlphaLISA technology. More recently we have developed high-content screening assays for tissue, cell and organelle morphology and protein expression and localization in 2D and 3D cultured cells and isolated primary cells using the Operetta high- throughput fluorescent confocal microscope with up to four different fluorescent stains. Additionally, sophisticated image analysis procedures are established to quantify tissue, cell and organelle morphology and protein expression and localization and high throughput processing procedures to prepare images for custom analysis by collaborators.

The DSC is under the direction of Dr. Ranjala Ratnayake (CTHR) who provides free consultation to UFHCC members. Additional services in drug development and screening at UF are available here.