CRISPR technology facilitates the precise editing of targeted regions of mammalian genomes and remains one of the key recent advances in biotechnology.
The CRISPR Functional Screening Shared Resource (CFS-SR) is located within the ICBR, offers UFHCC researchers with access to expertise in Cas9-mediated gene engineering in mammalian cells as well as custom services for CRISPR-based whole-genome phenotypic screens.
The CFS-SR supports functional screening for UFHCC members through both general resource development and project/team specific assistance.
Mission
To provide the capabilities and expertise necessary to successfully utilize genome wide and targeted CRISPR screening approaches.
Services
| CRISPR Services | Academic | Non-Profit | Commercial |
|---|---|---|---|
| CRISPR Project consultation (1 hour) | $0.00 | $0.00 | $0.00 |
| CRISPR Project consultation (additional hours) | $50.00 | $57.50 | $62.50 |
| Cell Line Optimization for Transduction or Nucleofection | $50.00 | $57.50 | $62.50 |
| CRISPR Single Cell KO in Cell Line Pool Using 1 sgRNA | $400.00 | $460.00 | $500.00 |
| CRISPR Single Cell KO in Cell Line Pool Using 3 sgRNAs (Recommended) | $1,000.00 | $1,150.00 | $1,250.00 |
| Generation of CRISPR Single Gene KO Stable Cell Line Using 3 sgRNAs [3 Clonal Isolates per Gene] | $2,500.00 | $2,875.00 | $3,125.00 |
| Vector-Free CRISPR Single Gene KO Stable Cell Line Using Nucleofection of Cas9 Protein and 3 sgRNA [3 Clonal Isolates] | $2,100.00 | $2,415.00 | $2,625.00 |
| Vector-Free CRISPR Single Gene KO Cell Line Pool Using Nucleofection of Cas9 Protein and 1 sgRNA [3 Clonal Isolates] | $700.00 | $805.00 | $875.00 |
Services can be accessed using the ICBR iLab web portal.
Leadership
Christopher Vulpe, MD, PhD
Director, CRISPR Functional Screening Shared Resource
