Mission
The UF Health Cancer Center CRISPR Functional Screening (CSF) developing shared resource provides cancer researchers with access to the expertise and tools to apply CRISPR-based genome editing approaches to enable functional screening of diverse biological systems relevant to cancer research.
Shared Resource Aims
- Provide consultation on study design of CRISPR-based functional screening approaches in cancer research
- Produce screen-ready stocks of selected genome-wide and targeted screen-ready CRISPR libraries in standard vector systems
- Construct custom CRISPR libraries for investigator-defined targeted gene sets
- Enable functional screening through development of standard protocols (SOPs), training, and technical assistance in implementation of screening methods
- Work collaboratively with investigators in the development and troubleshooting of novel approaches and innovative methodology at the forefront of screening technologies
- Coordinate routine NGS and bioinformatic analysis of functional screening data sets
Services available for Cancer Center members
Please use the support request form and our team will contact you to schedule a meeting.
- Consultation on mammalian CRISPR projects, including grant applications
- Provide letters of support for internal and external grant applications
- Facilitate individual CRISPR KO studies in mammalian cells by assisting in:
- Constitutive CRISPR KO in mammalian cells using Lentiviral vector systems
- Inducible CRISPR KO in mammalian cells using Lentiviral vector systems
- Facilitate CRISPR screening projects in mammalian cells by:
- Providing lentivirus packaged stocks of CRISPR screening libraries
- Optimizing lentivirus transduction of investigator provided mammalian cells
- Transducing mammalian cells and providing mutant cell library to investigators for carrying out in vitro and in vivo CRISPR screens
- Providing SOP, protocols, and troubleshooting for carrying out CRISPR screens
Screen-ready stocks of CRISPR libraries
CFS provides screen-ready genome-wide CRISPR libraries for selected publicly available genome wide CRISPR libraries, targeted CRISPR libraries, as well as several custom in-house developed libraries. While the focus is on human CRISPR libraries, several non-human resources have been developed and are available for use.
CRISPR Functional Screening Shared Resource
Custom CRISPR libraries
The facility can construct targeted CRISPR libraries for project-driven goals of research teams in appropriate vectors. Please consult with the facility about the process and timeline. Previously generated custom libraries available for UF researchers are listed above.
Bioinformatics Analysis
The functional screening facility works closely with the Cancer Center’s Biostatistics & Computational Biology Shared Resource to provide necessary computational support for effective use of CRISPR screens. Submit a request for bioinformatics support.
Leadership
Christopher Vulpe MD, PhD
Julia Bartel
Abderrahmane Tagmount
