CRISPR Functional Screening Shared Resource

gene editingCRISPR technology facilitates the precise editing of targeted regions of mammalian genomes and remains one of the key recent advances in biotechnology.

The CRISPR Functional Screening Shared Resource (CFS-SR) is located within the ICBR, offers UFHCC researchers with access to expertise in Cas9-mediated gene engineering in mammalian cells as well as custom services for CRISPR-based whole-genome phenotypic screens.

The CFS-SR supports functional screening for UFHCC members through both general resource development and project/team specific assistance.

Mission

To provide the capabilities and expertise necessary to successfully utilize genome wide and targeted CRISPR screening approaches.

Services

CRISPR Services Academic   Non-Profit  Commercial
CRISPR Project consultation (1 hour) $0.00 $0.00 $0.00
CRISPR Project consultation (additional hours) $50.00 $57.50 $62.50
Cell Line Optimization for Transduction or Nucleofection $50.00 $57.50 $62.50
CRISPR Single Cell KO in Cell Line Pool Using 1 sgRNA $400.00 $460.00 $500.00
CRISPR Single Cell KO in Cell Line Pool Using 3 sgRNAs (Recommended) $1,000.00 $1,150.00 $1,250.00
Generation of CRISPR Single Gene KO Stable Cell Line Using 3 sgRNAs [3 Clonal Isolates per Gene] $2,500.00 $2,875.00 $3,125.00
Vector-Free CRISPR Single Gene KO Stable Cell Line Using Nucleofection of Cas9 Protein and 3 sgRNA [3 Clonal Isolates] $2,100.00 $2,415.00 $2,625.00
Vector-Free CRISPR Single Gene KO Cell Line Pool Using Nucleofection of Cas9 Protein and 1 sgRNA [3 Clonal Isolates] $700.00 $805.00 $875.00

Services can be accessed using the ICBR iLab web portal.

Leadership