CRISPR technology facilitates the precise editing of targeted regions of mammalian genomes and remains one of the key recent advances in biotechnology.
The CRISPR Functional Screening Shared Resource (CFS-SR) is located within the ICBR, offers UFHCC researchers with access to expertise in Cas9-mediated gene engineering in mammalian cells as well as custom services for CRISPR-based whole-genome phenotypic screens.
The CFS-SR supports functional screening for UFHCC members through both general resource development and project/team specific assistance.
To provide the capabilities and expertise necessary to successfully utilize genome wide and targeted CRISPR screening approaches.
|CRISPR Project consultation (1 hour)||$0.00||$0.00||$0.00|
|CRISPR Project consultation (additional hours)||$50.00||$57.50||$62.50|
|Cell Line Optimization for Transduction or Nucleofection||$50.00||$57.50||$62.50|
|CRISPR Single Cell KO in Cell Line Pool Using 1 sgRNA||$400.00||$460.00||$500.00|
|CRISPR Single Cell KO in Cell Line Pool Using 3 sgRNAs (Recommended)||$1,000.00||$1,150.00||$1,250.00|
|Generation of CRISPR Single Gene KO Stable Cell Line Using 3 sgRNAs [3 Clonal Isolates per Gene]||$2,500.00||$2,875.00||$3,125.00|
|Vector-Free CRISPR Single Gene KO Stable Cell Line Using Nucleofection of Cas9 Protein and 3 sgRNA [3 Clonal Isolates]||$2,100.00||$2,415.00||$2,625.00|
|Vector-Free CRISPR Single Gene KO Cell Line Pool Using Nucleofection of Cas9 Protein and 1 sgRNA [3 Clonal Isolates]||$700.00||$805.00||$875.00|
Services can be accessed using the ICBR iLab web portal.